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Effect of increasing concentrations of Gua+ on the observed third-order rate constants (kcat/KGAKX)obs (M–2 s–1, Figure 2) for dianion activation of R269A mutant hlGPDH-catalyzed reduction of GA: solid symbols, [GA]carbonyl = 3.6 mM; open symbols, [GA]carbonyl = 1.8 mM. The slopes of these linear correlations are the fourth order rate constants kcat/KGAKXKGua reported in Table 1.
