FIG. 1.

Schematic of the LPS of wild-type and mutant B. pertussis and B. bronchiseptica. B. pertussis LPS has lipid A, a branched-core oligosaccharide, and a nonrepeating trisaccharide composed of GlcNAc, diNAcManA, and FucNAcMe. Full-length band A LPS (A) and truncated band B LPS (B), lacking the trisaccharide, are indicated. The WlbG mutant of B. pertussis fails to add the trisaccharide and only produces band B LPS. The WaaF mutant lacks the terminal trisaccharide, has only a single heptose in the core, and fails to react with the band B antibody. B. bronchiseptica LPS is similar to that of B. pertussis but has an additional repeating O-antigen homopolymer of 2,3-di-NAcGalA and palmitoylated lipid A. The PagP mutant fails to palmitoylate lipid A. The Wbm mutant of B. bronchiseptica has a deletion of the wbmB to wbmE genes, fails to add the O antigen, and produces band A LPS similar to that of wild-type B. pertussis. The Wlb mutant of B. bronchiseptica has a deletion in the operon required for the addition of the trisaccharide. Failure to add the trisaccharide appears to prevent addition of the O antigen, and the LPS produced by the Wlb mutant of B. bronchiseptica is similar to the band B LPS produced by the WlbG mutant of B. pertussis.