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LPS production by wild-type and mutant B. pertussis and B. bronchiseptica. (A) LPS was separated by SDS-PAGE gels and silver stained. O antigen and bands A and B are indicated. (B) Bacterial cells were separated by SDS-PAGE and transferred to PVDF membranes. Band A, immunoblot probed with monoclonal antibody to band A; band B, immunoblot probed with monoclonal antibody to band B. B. pertussis WT, wild-type strain BP338; B. bronchiseptica WT, wild-type strain RB50; Bvg−, strain RB54.
