FIG. 7.

Effects of SP-D on internalization of B. pertussis by rat alveolar macrophages. Bacteria alone or bacteria incubated with SP-D (10 μg/ml) were added at an MOI of 5 to alveolar macrophages from adult Harlan rats, and phagocytosis was allowed to occur for 1 h. The number of internalized bacteria was determined by fluorescence microscopy. Samples were prepared in triplicate for each experiment, and at least three independent experiments were performed. Data represent means ± standard errors of the means.