(a) Immunoblots showing changes in secretion of specific proteins in ETT2 regulator mutants. The antibodies used are indicated at the bottom. Lane M, molecular weight markers; lane S, parent strain Sakai 813; lane LZ2, ΔetrA ETT2 regulator mutant LZ2; lane LZ3, ΔeivF ETT2 regulator mutant LZ3. (b) SDS-PAGE gel showing complementation of secretion phenotype of the ΔetrA ETT2 regulator mutant. Lane M, molecular weight markers; lane S, parent strain Sakai 813; lane LZ2, ΔetrA ETT2 regulator mutant LZ2; lane LZ2c, complemented mutant strain LZ2/pBADetrA. Molecular sizes in kilodaltons are indicated on the left.