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. 2016 Mar 25;44(13):6102–6112. doi: 10.1093/nar/gkw193

Figure 4.

Figure 4.

Inhibition of histone H3T3 phosphorylation allows for re-association of the PHD finger-containing proteins with telophase chromatin. (A, B) Random cycling RPE-1 cells were incubated with 1 μM CHR6494 for 24 h and labelled as in Figure 3. Control cells were treated with DMSO solvent only. Histone modifications are depicted in green, DNA in blue and the PHD finger proteins in red. Pro- and telophases for the PHD finger proteins tested are shown in Supplementary Figure S5. (C) RPE-1 cells were incubated with 2 μM CHR6494 for 24 h and labelled as in (A). (D, E) RPE-1 cells were treated as under (A), and labelled with antibodies against DIDO (red) and either H3T6ph (green, D) or H3T3ph (green, E). DNA was counterstained with DAPI (blue). DIDO re-association with chromatin was observed in partially decondensed regions. Three right panels in (E) show magnification of the boxed area in the left panel. Scale bars, 5 μm.