Skip to main content
. 2016 Mar 25;44(13):6113–6126. doi: 10.1093/nar/gkw194

Figure 3.

Figure 3.

Sequence-specific binding of Fhl1 FH domain to RPG promoters. EMSA analyses performed to evaluate the association of purified Fhl1 FH domain (FHD) with the promoter region of RPL4B (A), RPP1A (B), RPS22B (C) and RPS28B (D). In each of these cases, wt and mutant (Fmut, altered in the Fhl1 binding site) promoter region fragments were compared for their ability to associate with increasing concentrations of FHD (indicated above the gel images). The wt and mutated sequences of Fhl1 binding site for each probe are indicated on the top of each panel. The results of EMSA evaluating the association of FHD with RPL28 (positive control) and FLR1 (non-specific DNA, negative control) promoter regions are shown in panels E and F, respectively, in comparison with binding to RPS22B promoter. The promoter DNA:FHD molar ratio is reported below each lane. (#) Free radiolabelled probe; (##) FHD-probe shifted complex; (###) supershifted complex likely corresponding to DNA fragments bound by two FHD molecules.