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. 2016 Mar 25;44(13):6113–6126. doi: 10.1093/nar/gkw194

Figure 5.

Figure 5.

Abf1and Fhl1 role and interactions at the RPS22B promoter. Abf1 and Fhl1 binding sites were mutated alone (Amut, Fmut) or in combination (AFmut) within the promoter region of RPS22B both in a TAP-tagged ABF1 and in a 13myc-tagged FHL1 strain. For each promoter mutant, (A) Abf1- and (B) Fhl1-promoter association was assessed by ChIP-qPCR. (C) Schematic representation of RPS22B transcription unit. (D) RPS22B and (E) SNR44 expression levels measured in Abf1-TAP wt and derivative strains carrying mutated Abf1, Fhl1 and Tbf1 binding sites, alone (Amut, Fmut, Tmut) or in combination (AFmut, TAFmut). Experiments in panels A, B, D and E were performed in triplicate. Data are represented as mean ± SEM. A one-way ANOVA test was used to compare the means of measurements for the set of strains in each experiment. (*) P < 0.05, (**) P < 0.01, (***) P < 0.001 using a Tukey post-hoc test.