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. 2016 Mar 25;44(13):6113–6126. doi: 10.1093/nar/gkw194

Figure 6.

Figure 6.

Response of Abf1-dependent RPG promoters to TOR pathway inactivation. (A–D) Exponentially growing, ABF1 TAP-tagged yeast strains, carrying either wt or promoter-mutated RP genes were treated with rapamycin (200 ng/ml). After 30 or 60 min of treatment, cells were collected for expression and Abf1 ChIP analyses of RPP1A (A, C) and RPS28B (B, D) genes. Expression levels are reported as relative to untreated wt cells; Abf1 enrichment for each strain is reported as relative to untreated cells of the same strain (t0). Data were collected from three independent replicates and are presented as mean ± SEM. (E) An exponentially growing, IFH1 TAP-tagged strain was treated with rapamycin (200 ng/ml). After 30 or 60 min of treatment, cells were collected for Ifh1 ChIP analysis of the indicated RPGs. Ifh1 enrichment for each gene is reported relative to the association measured in untreated cells (t0). Data were collected from three independent replicates and are presented as mean ± SEM. Data relative to RPL4B, RPP1A, RPS28A and RPS28B (see text) are not reported in the figure, as no significant enrichment over HHT2 was observed even at t0.