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. 2016 Apr 1;44(13):6157–6172. doi: 10.1093/nar/gkw209

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Vps75 associates with histones H3–H4 in multiple oligomeric assemblies. SEC-MALS analysis of DTSSP cross-linked Vps75 and Vps75 with histones H3–H4. (A) SEC-MALS analysis of Vps75 (5 nmol) cross-linked with DTSSP (100 nmol) at 200 mM NaCl, 20 mM HEPES pH 7.5 and eluted from Superdex 200 10/300 GL column (GE Healthcare) at 400 mM NaCl, 20 mM HEPES. Dashed line indicates the molecular weights at the peak elution volume peak elution volume. Fraction numbers are indicated in blue. (B) SDS-PAGE analysis (4–12% Bis–Tris NuPAGE gels) of fractions from the SEC-MALS analysis of cross-linked Vps75 and fractions treated with DTT. (C) Details of DTSSP cross-linker. (D) SEC-MALS analysis of Vps75 (5 nmol) and H3–H4 (5 nmol) cross-linked as described in (A) with fraction numbers are indicated in red. (E) SDS-PAGE analysis of fractions from the SEC-MALS analysis of cross-linked Vps75 H3–H4 as described in (B).