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. 2016 Apr 21;44(13):6391–6397. doi: 10.1093/nar/gkw303

Figure 3.

Figure 3.

Analysis of complementation of splicing activity in U1ΔNE using various antisera for selection of Gal3–U1 snRNP particles. Splicing reactions were carried out in the presence or absence of immunoprecipitates as indicated by the + or – signs above the bold solid line. The presence of NE or U1ΔNE in the splicing reaction mixture is indicated by the + or – signs below the bold solid line. Lane 1: 4 μl NE in a splicing assay volume of 12 μl, all of which were subjected to gel analysis. For lanes 2–5, the total splicing assay volume was 24 μl, half of which were subjected to gel analysis. Lane 2: 8 μl U1ΔNE and beads from anti-Gal3 (αGal3) rabbit #49 precipitation of 60% Buffer D (60%D). Lane 3: 8 μl U1ΔNE and beads from αGal3 rabbit #49 precipitation of Fr 3–5. Lane 4: 8 μl U1ΔNE and beads from αGal3 rabbit #24 precipitation of Fr 3–5. Lane 5: 8 μl U1ΔNE and beads from precipitation of Fr 3–5 by preimmune serum of rabbit #49. Arrows point to products of the splicing reaction.