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. 2016 Jun 13;44(13):6363–6376. doi: 10.1093/nar/gkw518

Figure 3.

Figure 3.

Long slow diffusion events by SA1 on DNA depend on the presence of telomeric sequences. (A) Dynamics of the full length Flag-SA1 on DNA tightropes containing centromeric sequences. Top: a schematic drawing of the ligated DNA substrate containing centromeric sequences. Bottom: a kymograph showing a mobile QD-labeled Flag–SA1 complex on the centromeric DNA tightrope. The scale bar (y-axis): 1 μm. (B) Comparison of the static and mobile SA1 populations on telomeric, genomic and centromeric DNA tightropes. The percentages of static SA1 molecules are 36% (N = 84), 11% (N = 63) and 4% (N = 97) on telomeric, genomic and centromeric DNA, respectively. (C) The percentages of SA1 (out of all mobile molecules) with long slow diffusion events (>2 s) on telomeric, genomic and centromeric DNA. The mean values are 51.2% (N = 54), 17.6% (N = 56) and 24.1% (N = 93), for telomeric, genomic and centromeric DNA substrates, respectively (from two or three independent experiments). The error bars represent SEM.