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. 2016 Jun 13;44(13):6363–6376. doi: 10.1093/nar/gkw518

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — The AT-hook motif in SA1 mediates non-specific DNA binding and subdiffusive dynamics on telomeric T270 DNA substrate. (A) A representative AFM image showing SA1-N (white arrow) binding to T270 DNA. The purple arrows point to the estimated boundaries of the telomeric region. (B) QD conjugation strategy: an antibody-sandwich method for Sumo-tagged SA1-N. (C) Kymographs of QD-labeled SA1-N (green, top panel) and full length SA1 R37A R39A (red, bottom panel) on T270 DNA tightropes. Scale bars (y-axis): 1 μm. (D) Fluorescence anisotropy experiments showing concentration-dependent binding of 3xFlag-tagged SA1 R37A R39A to DNA substrates containing 7 TTAGGG repeats (Tel7, left panel) or scrambled DNA sequences (right panel). The data were from two independent experiments.