Table 1. Summary from analysis of diffusion dynamics of QD-labeled full length WT SA1, SA1-N and SA1 R37A R39A on DNA tightropes.
| WT SA1-QDs | SA1-N-QDs | SA1 R37A R39A | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| DNA | D (μm2/s) | Alpha factor | N | % | D (μm2/s) | Alpha factor | N | % | D (μm2/s) | Alpha factor | N |
| T270 | 0.04 ± 0.01 (0.02 to 0.06) | 0.69 ± 0.03 (0.63–0.75) | 48 | 51.2% (N = 54) | 0.06 ± 0.01 (0.04–0.08) | 0.74 ± 0.03 (0.68–0.80) | 103 | 74.3% (N = 88) | 0.03 ± 0.01 (0.01–0.05) | 0.79 ± 0.05 (0.69–0.89) | 33 |
| Genomic | 0.14 ± 0.03 (0.08 to 0.20) | 0.89 ± 0.02 (0.85–0.93) | 36 | 17.61% (N = 55) | 0.12 ± 0.02 (0.08–0.16) | 0.89 ± 0.02 (0.85–0.93) | 160 | 38.6% (N = 103) | |||
| Centromeric | 0.11 ± 0.02 (0.07–0.15) | 0.82 ± 0.02 (0.78–0.86) | 75 | 24.1% (N = 93) | 0.03 ± 0.01 (0.01 to 0.05) | 0.83 ± 0.03 (0.77–0.89) | 36 | ||||
Note: D: diffusion constant (mean ± SEM).
%: percentage of mobile molecules with long slow diffusion events (>2 s).
N: numbers of molecules analyzed for diffusion constant and alpha factors calculations.
The numbers in the parenthesis represent confidence intervals at the 95% confidence level.