Table 1. Transcript and protein levels, shown as female/male ratio, of known marker-proteins of Plasmodium female (FG) or male (MG) gametocytes.
| gene ID | Product | P. falciparumA | P. bergheiB | Marker | |
|---|---|---|---|---|---|
| FG/MG protein ratio | FG/MG transcript ratio | FG/MG protein ratio | |||
| PF3D7_1250100 | Pfg377 (51) | 5.9E+03 | 3.8E+01 | 4.9E+01 | FG |
| PF3D7_1346800 | 6-cysteine protein (P47) (54) | 2.2E+03 | 2.2E+01 | 1.7E+01 | FG |
| PF3D7_1475500 | LCCL protein (CCp1) (15) | 2.1E+03 | 2.3E+01 | 6.1E+00 | FG |
| PF3D7_1426500 | ABC transporter, (EPP family)(53) | 1.5E+03 | 4.4E+01 | N.D | FG |
| PF3D7_1407000 | LCCL protein (CCp3) (9,15) | 8.1E+02 | 7.4E+00 | 6.8E+00 | FG |
| PF3D7_0719200 | NIMA related kinase 4 (NEK4)(9) | 9.7E+01 | 2.8E+01 | 2.0E+01 | FG |
| PF3D7_1474900 | trailer hitch homolog (CITH) (11) | 9.7E+01 | 1.6E+00 | 1.1E+01 | FG |
| PF3D7_0320800 | ATP-dep.RNA helicase (DOZI) (10) | 5.3E+01 | 3.1E+00 | 1.1E+01 | FG |
| PF3D7_0729900 | dynein heavy chain, putative (9) | 2.3E+01 | 5.9E-01 | 2.3E+00 | FG |
| PF3D7_0415600 | adenylate kinase (GAK) (50) | 7.0E+00 | 1.2E+01 | N.D | FG |
| PF3D7_0525900 | NIMA related kinase 2 (NEK2) (52) | 4.6E+00 | 2.7E+01 | N.D | FG |
| PF3D7_1031000 | Pfs25 (15) | 2.3E+00 | 3.6E+01 | N.D | FG |
| PF3D7_0422300 | alpha tubulin 2 (9) | 7.6E-01 | 2.5E-02 | 2.5E-01 | MG |
| PF3D7_1441400 | FACT-S (48) | 4.0E-01 | 2.0E-01 | 6.1E-02 | MG |
| PF3D7_0517400 | FACT-L (9,48) | 1.7E-01 | 1.8E-01 | 1.4E-02 | MG |
| PF3D7_1412500 | actin II (ACT2) (42) | 1.5E-01 | 1.6E+00 | 1.1E-01 | MG |
| PF3D7_1113900 | MAP2 protein kinase (9,49,50) | 9.1E-02 | 8.6E-02 | 1.1E-01 | MG |
| PF3D7_1014200 | HAP2 (9,47) | 8.3E-02 | 1.5E-02 | N.D | MG |
| PF3D7_0717500 | CDPK4 protein kinase (50) | 7.9E-02 | 3.8E-02 | 1.4E-01 | MG |
| PF3D7_0302100 | SRPK1 protein kinase (50) | 6.6E-02 | 1.7E-01 | N.D | MG |
| PF3D7_1216700 | perforin like protein 2 (PPLP2) (43) | 6.0E-02 | 2.2E-01 | N.D. | MG |
| PF3D7_1122900 | dynein heavy chain, putative (9) | 5.6E-02 | 1.1E-02 | 1.9E-01 | MG |
| PF3D7_0905300 | dynein heavy chain, putative (9) | 5.5E-02 | 2.8E-03 | 1.3E-01 | MG |
| PF3D7_1023100 | dynein heavy chain, putative (9) | 5.1E-02 | 2.6E-02 | 1.6E-01 | MG |
| PF3D7_1228300 | NEK1 protein kinase (44) | 4.1E-02 | 1.4E-01 | 4.7E-01 | MG |
| PF3D7_1465800 | dynein beta chain, putative (9) | 3.3E-02 | 8.4E-02 | 5.7E-01 | MG |
| PF3D7_0111000 | kinesin, putative (9) | 2.0E-02 | 9.1E-02 | 1.6E-01 | MG |
| PF3D7_0208900 | 6-cysteine protein (P230p) (45) | 1.9E-02 | 1.9E-02 | 6.3E-01 | MG |
A: Determined in this study; B: Proteome evidence of the P. berghei orthologs; based on reprocessed proteome data from Khan et al. Cell, 121, 675-687 (2005). Quantification of P. berghei protein levels was performed in this study; N.D.: Not Detected ; Gene ID underlined: reporter markers for constructs purified by FACS.