Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jun 24;44(13):6318–6334. doi: 10.1093/nar/gkw565

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 7. — Importance of the DDX6-4E-T interaction for miRNA-mediated repression. (A) Schematic cartoon depicting the components of the miRNA reporter assay. (B) HeLa cells were treated with control (β-globin) or 4E-T siRNA, and transfected with HA-4E-T (wt and deletion forms), RL-Hmga2 (wt or let-7 binding site) mutant and control FL plasmids. Assays were performed in the presence of the dominant negative PAN2_catal and CAF1_catal to eliminate effects resulting from mRNA deadenylation (33). Lysates were analysed by Dual luciferase luminometry. (***P < 0.001, *P <0.05, ns not significant, two tailed t test; (n = 3–4)). (C) Western blotting of transfected samples.