FIG. 5.

Expansion and activation of DO11 T cells in IFN-γ^−/− animals. FACS analysis of lymph nodes from DO11-transferred, immunized IFN-γ^−/− mice infected with parental Prugniaud parasites (top left) or P30-OVA-transgenic cells (bottom left) is shown. Animals infected with parasites secreting P30-OVA developed a substantial KJ126⁺ CD4⁺-T-cell population (red box), which was analyzed for proliferation based on CFSE staining (top right) and for activation based on CD62L expression (bottom right).