Table 4. The primer sequences and their polymorphism assessment results.
Locus name | Primer sequences (5′−3′) | Ta(°C) | Observed no. of alleles | Size range(bp) | N | HObs | HExp | PIC |
---|---|---|---|---|---|---|---|---|
YPM 6 | CAGTCGGGCGTCATCAAAGCTGATAGGCGTGCTGAT | 55 | 4 | 187–208 | 32 | 0.156 | 0.205 | 0.195 |
ACTTTAAAACACAGGGACAGGTCT | ||||||||
YPM27 | CAGTCGGGCGTCATCAATTGGGCCTGCAGTTATTTT | 55 | 8 | 108–205 | 25 | 0.92 | 0.876 | 0.842 |
TGTGAGTGTGTGAGTGTGAGTGT | ||||||||
YPM41 | CAGTCGGGCGTCATCACCCACCTGCAGAACACTACA | 55 | 4 | 210–231 | 31 | 0.387 | 0.342 | 0.32 |
GTTTAACCCCACGAAAATGC | ||||||||
YPM30 | CAGTCGGGCGTCATCAGACACGGAAATGGAAAAAGGT | 55 | 11 | 174–210 | 31 | 1 | 0.705 | 0.645 |
CCAGCTGGAGAAACAGGACT | ||||||||
YPM20 | CAGTCGGGCGTCATCAGGACCACTCGTTTTGCTCTG | 55 | 6 | 246–276 | 31 | 0.548 | 0.499 | 0.464 |
CACAAACACATGCACACTCG | ||||||||
YPM34 | CAGTCGGGCGTCATCAGTTATCATGCTGGGGAGGAC | 55 | 2 | 190–206 | 32 | 0.406 | 0.329 | 0.271 |
CAAACCTCATCACAGGAGCA | ||||||||
YPM46 | CAGTCGGGCGTCATCAGCTAAAAGAAACTCCTGGCAAA | 55 | 14 | 173–218 | 28 | 0.929 | 0.89 | 0.861 |
GCGGATCAAACGAAAGAAAA | ||||||||
YPM17 | CAGTCGGGCGTCATCACCTGCCTCCTGAACCTTTCT | 55 | na | na | na | na | na | na |
TGCTACGACACTGCCTGCTA |