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. 2017 Feb 3;12(2):e0171606. doi: 10.1371/journal.pone.0171606

Table 1. Oligonucleotide primers used for cloning of the CpGH31 CBM32s.

Primer Sequence
CBM32-1 (FWD) 5’-GTAAATAAGGATGGACATTC-3’
CBM32-1 (REV) 5’-TCACTTATAAGGTCTAAACTCTC-3’
CBM32-2 long (FWD) 5’-CTGGTTCCGCGTGGATCCGCTAAAATTATTGATTT AGAAAGTG-3’
CBM32-2 long (REV) 5’-CCGCTCGAGCTTCTCATAAAATTCAATTTC-3’
CBM32-2 short (FWD) 5’-GGAATTCCATATGAACATTACTGTAAGTGGTG-3’
CBM32-2 short (REV) 5’-CCGCTCGAGTTATTCACTCTTCTCATAAAATTC-3’
CBM32-3 (FWD) 5’-GATTCTAGCAAGTTAGAAGC-3’
CBM32-3 (REV) 5’-TCATTTTCCGTAGAATACTAATTC-3’