Table 1. Oligonucleotide primers used for cloning of the CpGH31 CBM32s.
Primer | Sequence |
---|---|
CBM32-1 (FWD) | 5’-GTAAATAAGGATGGACATTC-3’ |
CBM32-1 (REV) | 5’-TCACTTATAAGGTCTAAACTCTC-3’ |
CBM32-2 long (FWD) | 5’-CTGGTTCCGCGTGGATCCGCTAAAATTATTGATTT AGAAAGTG-3’ |
CBM32-2 long (REV) | 5’-CCGCTCGAGCTTCTCATAAAATTCAATTTC-3’ |
CBM32-2 short (FWD) | 5’-GGAATTCCATATGAACATTACTGTAAGTGGTG-3’ |
CBM32-2 short (REV) | 5’-CCGCTCGAGTTATTCACTCTTCTCATAAAATTC-3’ |
CBM32-3 (FWD) | 5’-GATTCTAGCAAGTTAGAAGC-3’ |
CBM32-3 (REV) | 5’-TCATTTTCCGTAGAATACTAATTC-3’ |