Fig 4. Activators of TRPV1 induce neither an increase of [Ca2+]i nor degranulation.
PMCs were obtained from WT mice. (A-C) Time course of [Ca2+]i changes in Fura-2 loaded PMCs stimulated with 10μM Capsaicin (A), protons, pH = 5.5 (B), and hypertonic solution, +50 mOsm kg–1 (C). 1 μg/ml 48–80 was used as a positive control. Bar graph shows the percentage of β-hexosaminidase release. SD- Spontaneous degranulation, Iono- 10μM Ionomycin, 48-80- 50μg/ml Compound 48–80, and Cap- 10μM Capsaicin. Graphs represent the mean ± SEM of at least 3 independent preparations.