Fig 6. Activator of TRPV4 induces neither an increase of [Ca2+]i nor degranulation.
PMCs were obtained from WT mice. (A) Time course of [Ca2+]i changes in Fura-2 loaded PMCs stimulated with 10μM GSK-1016790A. 1 μg/ml 48–80 was used as a positive control. (B) Bar graph shows the percentage of β-hexosaminidase release. SD-Spontaneous degranulation, Iono- 10μM Ionomycin, 48-80- 50μg/ml Compound 48–80, and GSK- 10μM GSK-1016790A. Graphs represent the mean ± SEM of at least 3 independent preparations.