Fig 7. Heat triggers an increase of [Ca²⁺]_i and degranulation independently of TRPV2.

PMCs were obtained from WT mice and mice deficient for TRPV2 (red). (A-B) Time course of [Ca²⁺]_i changes in Fura-2 loaded PMCs stimulated with heat. Representative single cell traces of [Ca²⁺]_i changes in response to a temperature ramp (from 20–50°C). Mean values of [Ca²⁺]_i elevation triggered by heat (50°C). 1 μg/ml 48–80 was used as a positive control. (C) Bar graph shows the percentage of β-hexosaminidase release. SD-Spontaneous degranulation, Iono- 10μM Ionomycin, 48-80- 50μg/ml Compound 48–80, and heat (incubation at 52°C for 5–30 minutes). Graphs represent the mean ± SEM of at least 3 independent preparations. Temperature color code in legend corresponds to colors in horizontal bar, but not to those in cell traces.