FIG. 2.

Classical MHC class I-unrestricted killing of R. equi-infected targets. PBMC from four horses were stimulated for 5 days with either (A) live virulent R. equi, (B) no antigen (medium alone), or (C) 10-μg/ml SRA. PBAC targets were infected with live virulent R. equi for 12 h or were uninfected. Four and a half hours prior to collection of the supernatants, effectors were added to targets at an E:T of 5:1 (A), 5:1 (B), or 20:1 (C). An asterisk indicates where lysis of infected targets is significantly increased (>3 standard errors compared to the corresponding uninfected control).