Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Construction of pJLE211, carrying the mrpH23-157-ctxA2 translational fusion and ctxB. A PCR fragment carrying mrpH23-157 was cloned into the SphI and ClaI restriction sites within the multiple cloning site (MCS) that is immediately upstream of ctxA2 (see text for details). IIb, LTIIb B gene leader sequence; pBAD, inducible arabinose promoter; pC, araC promoter; cat, chloramphenicol acetyltransferase; p15A ori, origin of replication; sd10, T7 gene 10 ribosome binding site.
