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. 2004 Dec;72(12):6852–6859. doi: 10.1128/IAI.72.12.6852-6859.2004

FIG. 4.

FIG. 4.

Infected horse plasma reduced dominance of the p44-18 transcript in cell culture. (A) Colony hybridization of p44 cDNA clones from A. phagocytophilum HZ incubated with horse (EQ005) day zero plasma (pre-tick attachment) and day 22 plasma at 6 days p.c. hvp44-18 and pan-p44 probes each were hybridized to one of the duplicated membranes each with 100 p44 cDNA clones. Two cDNA clones with p44-18 and p44-40 inserts were used as a positive and a negative control, respectively. A pCRII plasmid with a non-p44 insert was also used as negative control. Approximately 14% of p44 cDNA clones had the p44-18 insert with A. phagocytophilum incubated with day 22 p.t. plasma, and approximately 80% of p44 cDNA clones had the p44-18 insert with those incubated with day zero plasma. (B) Temporal changes in the p44-18 transcript population in A. phagocytophilum incubated with plasma obtained on day zero (preinfection) and day 31 p.t. from EQ005, FBS, or RPMI 1640 medium. Colony hybridization analysis was performed on 100 p44 cDNA clones in each specimen at each day p.c. to determine the percentage of cDNA clones with p44-18 inserts.