. 2004 Dec;72(12):6852–6859. doi: 10.1128/IAI.72.12.6852-6859.2004

TABLE 1.

Sequences of oligonucleotides used in RT-PCR, PCR, colony hybridization, and cloning

Primer set no.	Primer name	Target	Purpose	Size (bp)	5′ primer	3′ primer
1	p3709-p4257	p44 paralogs	C-PCR, RT-PCR	∼550	`GCTAAGGAGTTAGCTTATGA`	`AGAAGATCATAACAAGCATTG`
2	p3709-pCompI	p44 paralogs	Competitor construction	252	`GCTAAGGAGTTAGCTTATGA`	`CTGGGATCCACTACACTGCGACGTG^a`
3	pCompII-p4257	p44 paralogs	Competitor construction	221	`CGTGGATCCGGTAGATCGAGTAACAACAG^a`	`AGAAGATCATAACAAGCATTG`
4	pComp16SII-pComp16SI	A. phagocytophilum 16S rRNA	C-PCR	323	`CGGGGGAAAGATTTATCGCTATTA`	`CGCTTGCCCCCTCCGTATTA`
5	HB-ACTIN5-HB-ACTIN3	Horse β-actin	DNA PCR	628	`CTGGCACCACACCTTCTACAACGAG`	`GTACAGGTCCTTACGGATGTCGACG`
6	p5u37-p4257	p44 upstream sequence (p44 expression locus)	First-step DNA PCR	∼1,230	`CGTTATTTGTTCTAGAGAAAG`	`AGAAGATCATAACAAGCATTG`
7	p18-5′-p18-3′	p44-18 hv region	Colony hybridization	123	`GTGATAGTAGCAATGCTG`	`CATCACTCAATGTGTTAGAA`
8	p44con-p5247	p44 paralog C terminus conserved region	Colony hybridization	102	`AGTAGCAGGGTTACTAGC`	`AGAAGATCATAACAAGCATTG`
9	18RECS-18RECA	p44-18 hv region	rhvP44-18 cloning	271	`TCCGAATTCGGAGACAAAGCGGA`	`CGCAAGCTTCTATACAGCTTTGGC`

BamHI site is underlined.