TABLE 2.
Characteristics of p44 cDNA clone type distribution after incubation of A. phagocytophilum with horse EQ005 day 31 and day 0 plasma
| Days p.c. | Horse plasma | p44 clone type distributionb | No. of clone typesc | Entropyd |
|---|---|---|---|---|
| 2.5 | day 31 | 14, 3, 2, 2, 1, 1, 1 | 7 | 0.71 |
| day 0 | 18, 1, 1, 1 | 4 | 0.41 | |
| 6 | day 31 | 10, 3, 2, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1 | 13 | 0.82 |
| day 0 | 19, 2, 1, 1, 1, 1, 1, 1, 1 | 9 | 0.58 | |
| 12 | day 31 | 10, 4, 3, 2, 1, 1, 1, 1, 1, 1 | 10 | 0.82 |
| day 0 | 20, 2, 1, 1, 1 | 5 | 0.48 | |
| 12a | day 31 | 6, 4, 3, 3, 3, 3, 2, 2, 1, 1, 1 | 11 | 0.94 |
| day 0 | 18, 1, 1, 1, 1, 1, 1 | 7 | 0.52 |
a
Independent experiment.
b
Each series shows the number of clones comprising each clone type for the given sample.
c
Number of sequence indistinguishable cloned cDNAs.
d
Normalized Shannon entropy (H′), a calculated value that incorporated the number and distribution of clone types. A paired t test was used to compare the difference in entropy between samples of day 31 and day 0 horse plasma; P < 0.003.