FIG. 1.
T-cell recognition of mycobacterial antigens is dependent on the MHC haplotype. (A) Splenocytes from infected B10.BR, B10.A, 2R, 4R, 5R, and B10/J mice were cultured with or without M. tuberculosis antigens, and the amounts of IFN-γ released in the supernatants after 48 h were measured by ELISA. Each group contained six mice, and the data represent means ± standard deviations. (B) Amounts of IFN-γ in the BAL fluid were measured by ELISA. Data represent the means of six mice per group ± standard deviations. (C) At 3 weeks postinfection, splenocytes from C3H/HeJ (HeJ), C3H/HeSnJ (SnJ), and C3.SW-H2b/SnJ (C3Hb) mice were cultured with or without mycobacterial antigens and then assayed as described for panel A. (D) Comparison of IFN-γ production in the BAL fluid of C3H substrains at week 3 postinfection.