Table 6. Summary of biochemical and kinetic properties of PaDHPAO.
| Properties | Value | ||
|---|---|---|---|
| Biochemical properties | Metal content | Fe(II) (mole/subunit) | 0.71 |
| Molecular weight (kDa) | Subunit molecular weight | 30 | |
| Native molecular weight | 112.2 | ||
| Substrate specificity (%relative activity) | DHPA | 100 | |
| DHPP | 2.6 | ||
| DHB | 0.002 | ||
| Optimum pH | 7.5 | ||
| Optimum temperature (°C) | 55 | ||
| Transition temperature (°C) | 35 | ||
| Melting temperature (°C) | 50–55 | ||
| Kinetic properties | In the absence of ascorbic acid (assayed within 2 hour) | kcat (s-1) | 62.4 |
| Km, DHPA (μM) | 67 ± 7 | ||
| kcat/Km, DHPA (μM-1s-1) | 0.9 | ||
| In the presence of ascorbic acid | kcat (s-1) | 63.5 | |
| Km, DHPA (μM) | 58 ± 8 | ||
| kcat/Km, DHPA (μM-1s-1) | 1.1 | ||