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. 2004 Dec;72(12):7231–7239. doi: 10.1128/IAI.72.12.7231-7239.2004

FIG. 2.

FIG. 2.

Fluorescence microscopy (A and B) and TEM (C to E) of human monocyte-derived DC infected with C. trachomatis serovar E or L2. After human DC were infected with serovar E or L2 at an MOI of 10:1, DC were cultured for the indicated time periods and then fixed for fluorescence microscopy or TEM. (A) DC infected with serovar L2 for 24 h and stained with Chlamydia MOMP-specific antibody (green) and Evans blue (red). (B) DC infected with serovar E for 48 h and stained with Chlamydia MOMP-specific antibody (green) and Evans blue (red). (C and D) TEM of DC infected with serovar L2 for 24 h (C) and 48 h (D). (E) TEM of DC infected with serovar E for 72 h. Elementary bodies (EB) and reticulate bodies (RB) can be distinguished within the chlamydial inclusion.