FIG. 6.
Both viable and inactivated Chlamydia bacteria induce the production of pro-IL-1β by human monocyte-derived DC. DC (1.5 × 106) were infected with C. trachomatis serovar L2 (MOI, 10:1) (lane 2), pulsed with heat-inactivated serovar L2 (MOI, 10:1) (lane 3), or treated with 1 μg of LPS/ml (lane 4) as indicated. Untreated DC (lane 1) and mature recombinant IL-1β (lane 5) were used as controls. DC lysates were prepared 24 h after treatment, and the presence of IL-1β in DC lysates was analyzed by Western blotting. The results are representative of three independent experiments.