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Active caspase-1 is detected in human monocyte-derived DC after infection with viable but not heat-killed C. trachomatis. DC were either infected with C. trachomatis serovar L2 (L2) at MOIs of 10, 5, and 1:1 or pulsed with heat inactivated serovar L2 (HK L2) (MOI, 10:1). Intracellular levels of active caspase-1 were determined by FACS analysis in DC 24 h postinfection. Results of one representative experiment of five are shown.
