TABLE 1.
Viable and heat-inactivated EB from C. trachomatis serovar L2 induce the secretion of IL-6, IL-8, TNF-α, and IL-12p70 from human monocyte-derived DC, and only viable Chlamydia induces the secretion of IL-1β
| Treatment | Cytokine levela
|
||||
|---|---|---|---|---|---|
| IL-1β (pg/ml) | IL-6 (pg/ml) | IL-8 (ng/ml) | TNF-α (pg/ml) | IL-12p70b (pg/ml) | |
| Medium | <10 | <10 | 50 ± 10 | <10 | <10 |
| E. coli LPS | <10 | 2,100 ± 300 | 300 ± 50 | 14,000 ± 5,500 | 6,900 ± 4,800 |
| L2 | 2,000 ± 500 | 2,100 ± 300 | 300 ± 80 | 17,000 ± 5,200 | 3,200 ± 2,100 |
| HK L2 | <10 | 1,800 ± 400 | 300 ± 100 | 9,000 ± 6,000 | 1,700 ± 1,500 |
a
Monocyte-derived DC (2 × 105) were either infected as indicated with C. trachomatis serovar L2 (L2) treated with heat-inactivated L2 (HK L2) at an MOI of 10:1 or stimulated with 1 μg of LPS/ml. Cytokines were determined by ELISA in supernatants taken at 24 h posttreatment. Mean ± SD were calculated from the results obtained with five different donors.
b
IFN-γ was added to cultures at 10 ng/ml.