TABLE 2.
DC treatment | Cytokine level (pg/ml)a
|
||
---|---|---|---|
IL-1β | IL-6 | TNF-α | |
Medium | <10 | <10 | <10 |
L2 | 500 ± 100 | 1,400 ± 60 | 5,200 ± 200 |
HK L2 | <10 | 1,100 ± 100 | 2,300 ± 700 |
E | 300 ± 110 | 1,000 ± 20 | 7,300 ± 100 |
HK E | 70 ± 15 | 1,200 ± 50 | 3,300 ± 1,000 |
Monocyte-derived DC (2 × 105) were infected as indicated with C. trachomatis serovar L2 (L2) and serovar E (E) or treated with heat-inactivated L2 (HK L2) and heat- inactivated E (HK E) at an MOI of 20:1. Cytokine levels were determined by ELISA in supernatants taken at 24 h posttreatment. Means ± SD were calculated from the results obtained with two different donors.