TABLE 2.
Primers used for PCR amplification
| Primer | Purpose | Primer sequence (5′→3′)a |
|---|---|---|
| exoT5 | Amplification of exoT | ATATCCCATCGGGTTCTCCGCCCCGG |
| exoT-CM-2 | Amplification of exoT | CGTTTCGTTATGCAGGAAGC |
| exoSl-F | Amplification of exoS | TCTGAATTCTTCCAGGCGGGTGAACATCA |
| exoSl-R | Amplification of exoS | TTTAGATCTCACCCTGGTATCCAAGGCGA |
| 70.6 | Amplification of exoU | GCAGCCTATCGTGCAAG |
| 70.9 | Amplification of exoU | AGCGTTAGTGACGTGCG |
| pscJ-5′ | Amplification of pscJ | CAGTCTCGAAGAACTCTCCG |
| pscJ-3′ | Amplification of pscJ | TGCGCCGTACCCGCGCACCG |
| R146A-forward | Mutagenesis of exoS | CGGAGATGGGGCGCTGGCTTCGCTGAGCACCGC |
| R146A-reverse | Mutagenesis of exoS | GCGGTGCTCAGCGAAGCCAGCGCCCCATCTCCG |
| E379A/E381A-forward | Mutagenesis of exoS | CGAACTACAAGAATGCAAAAGCGATTCTCTATAACAAAG |
| E379A/E381A-reverse | Mutagenesis of exoS | TTGTTATAGAGAATCGCTTTTGCATTCTTGTAGTTCGAT |
a
Nucleotides altered for insertion of point mutations are in bold and underlined.