TABLE 2.
Plasmid constructs used in this study, characteristics of recombinant proteins, and binding properties of anti-PlpE antibodies, determined densitometrically
| Plasmid | Recombinant protein | Deleted region (amino acids) | Binding capacity of anti-PlpE antibodies
|
|
|---|---|---|---|---|
| Vola (INT/mm2) | % Binding | |||
| pSAC3 | rPlpE (pRSETA) | 0b | 2,472 | 100 |
| pSAC30 | rPlpEΔC106 | 232-337 | 2,333 | 94.3 |
| pSAC31 | rPlpEΔC96 | 242-337 | 2,450 | 99.1 |
| pSAC32 | rPlpEΔC86 | 252-337 | 2,635 | 106 |
| pSAC63 | rPlpEΔN28 | 1-28 | 2,528 | 102 |
| pSAC64 | rPlpEΔN76 | 1-76 | 699 | 28.0 |
| pSAC65 | rPlpEΔN150 | 1-150 | 310 | 12.5 |
| pSAC67 | rPlpE (pET28) | 0b | 2,472 | 100 |
a
Sum of the intensities of the pixels inside the volume boundary × area of a single pixel (in millimeters squared).
b
Mature rPlpe with 337 amino acids.