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. 2004 Dec;72(12):6799–6805. doi: 10.1128/IAI.72.12.6799-6805.2004

FIG. 2.

FIG. 2.

Antigen preparations of HBHA used as vaccines. (A) HBHA purified by heparin-Sepharose gradient chromatography (lane 1) or by subsequent reverse-phase HPLC (lane 2) was analyzed by SDS-PAGE, and gel lanes were stained with Coomassie blue. (B) Immunoblots containing HBHA purified by heparin-Sepharose gradient chromatography (lane 1) or by subsequent reverse-phase HPLC (lane 2), as well as recombinant His-tagged HBHA purified from M. smegmatis (lane 3), were probed with MAb D2, which recognizes methylated HBHA. The positions of the 28-kDa native HBHA protein and molecular mass standards are indicated on the left.