FIG. 6.

Effect of passive immunization with anti-HBHA monoclonal antibodies on the infection of mouse tissues with M. tuberculosis following aerosol challenge. C57BL/6 mice (five mice in each group) were inoculated intravenously with 250 μg each of IgG purified from MAb D2 and MAb E4 ascites (open bars) or with 250 μg each of IgG (isotype control) purified from MAb BP-G10 and MAb HB-65 ascites (striped bars) ∼24 h prior to aerosol challenge with 100 CFU of the M. tuberculosis Erdman strain. The bacterial burdens in lungs, spleens, and livers were determined by plating 28 days following challenge and compared with the burdens in mouse tissues that received no MAbs (solid bars). Statistical analyses were performed as described in Materials and Methods.