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. 2004 Dec;72(12):6914–6923. doi: 10.1128/IAI.72.12.6914-6923.2004

FIG. 3.

FIG. 3.

86Rb release from CaCo-2 cells induced by rCPE variants. Confluent CaCo-2 cells in 24-well plates were labeled with 4 μCi of 86Rb per well for 4 h and then treated with increasing concentrations of rCPE species. 86Rb released into the culture medium after 15 min of rCPE treatment was collected. To correct for background release, the data were converted to the percentage of maximal 86Rb release (see Materials and Methods). (A) Depicted are the following rCPE species: rCPE (♦), D45A (▴), D48A (○), I51A (□), and L52A (•). (B) 86Rb release experiments with rCPE saturation variants with the following rCPE species: rCPE (♦), D48A (○), D48E (▵), D48N (⋄), I51A (□), I51L (•), and I51V (▪). All data points represent means of three independent experiments, and error bars represent the standard deviations.