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. 2015 Dec 31;94(52):e2137. doi: 10.1097/MD.0000000000002137

FIGURE 6.

FIGURE 6

The activation of STAT3 signaling pathway in rhIL-22 treated FLS. The FLS were treated with 50 ng/mL rhIL-22 and/or 100 μM AG490 for different times. The representative Western blot chart and statistical analysis show tyrosine phosphorylation of STAT3 increased in a time-dependent manner when compared with controls (A, B) (∗∗∗P < 0.001). Simultaneously, a significant decrease in rhIL-22 stimulated tyrosine phosphorylation of STAT3 was found after treatment with the combination of AG490 when compared with single rhIL-22 stimulation (D, E) (∗∗∗P < 0.001). However, the total protein of STAT3 did not change when compared with controls (A, C, D, F) (P > 0.05). The results are representative of 3 independent experiments. FLS = fibroblast-like synoviocytes, rhIL-22 = recombinant human interleukin 22.