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. 2015 Dec 31;94(52):e2137. doi: 10.1097/MD.0000000000002137

FIGURE 7.

FIGURE 7

AG490 blocks the proliferation of FLS promoted by rhIL-22 or NKp44+NK cells culture supernatants. The FLS between passages 4 and 5 were treated by 100 μM AG490 combined with 50% NKp44+NK cell culture supernatants or 50 ng/mL rhIL-22 for 24, 48, or 72 hours, respectively. And then, they were analyzed by MTT assay. AG490 blocks the proliferation of RA-FLS interfered by rhIL-22 (A). AG490 blocks the proliferation of RA-FLS which were interfered with NKp44+NK cell supernatant (B). Values represent mean ± SD of O.D. 490 nm. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001. The data are representative of seven independent experiments. FLS = fibroblast-like synoviocytes, MTT = methyl thiazolyl tetrazolium, NK = natural killer, RA = rheumatoid arthritis, rhIL-22 =  recombinant human interleukin 22, SD = standard deviation.