FIG. 1.
Administration of IFN-γ to post-CLP mice attenuated elevated IL-10 production and increased serum concentrations of IL-12 and TNF-α after P. aeruginosa challenge. Mice were subjected to either CLP or sham surgery. Five days later, the mice were given intravenous injections of saline or 5 × 107 CFU of P. aeruginosa and were sacrificed 6 h later for collection of blood samples. Plasma cytokine concentrations were measured by enzyme-linked immunosorbent assay. Graphs show P. aeruginosa-induced plasma concentrations of (A) IFN-γ, (B) IL-10, C) IL-12, and D) TNF-α concentrations in sham and post-CLP mice. *, significantly different from sham group; #, significantly different from CLP group (sham group, n = 4; CLP group, n = 12; CLP + IFN group, n = 14).