FIG. 2.

Administration of IFN-γ caused increased numbers of CD3⁺ T cells and NK cells expressing IL-12R in post-CLP mice after Pseudomonas challenge. Mice were subjected to either CLP or sham surgery. Five days later, the mice were given intravenous injections of saline or 5 × 10⁷ CFU of P. aeruginosa, and they were sacrificed 6 h later. Spleens were aseptically excised and homogenized. Splenic homogenates were analyzed by flow cytometry after staining for cell surface markers. (A) Percentage of CD3⁺ T cells expressing IL-12R in control and IFN-γ-treated mice; (B) number of CD3⁺ IL-12R⁺ T cells as a percentage of all splenocytes; (C) percentage of NK cells expressing IL-12R; (D) number of NK1.1⁺ IL-12R⁺ T cells as a percentage of all splenocytes. *, significantly different from sham group; #, significantly different from CLP group (n = 6 to 10 per group).