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Body weight gain following vaccination with 3-1E protein. Chickens were vaccinated in ovo with PBS (0) or 25, 50, 100, or 500 μg of 3-1E and either not infected (−) or orally infected with 10,000 oocysts of E. acervulina (+) at 14 days posthatch. Body weights were determined at 0 and 6 days postinfection. Each bar represents the mean plus standard error of the mean (n = 5). The asterisks indicate significantly increased weight gains compared with the nonvaccinated and infected control.
