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. 2004 Dec;72(12):7247–7256. doi: 10.1128/IAI.72.12.7247-7256.2004

FIG. 1.

FIG. 1.

Hematoxylin and eosin staining of lung tissues at 2 h (A), 8 h (B), and 24 h (C) after aerosolization with LPS or at 24 h (D) after aerosolization with 0.9% saline. Note that infiltration of neutrophils into the air spaces and interstitial edema were evident in LPS-treated lungs in a temporal manner. The arrow indicates infiltrating inflammatory cells. Cytospin preparations of BALF cells stained with Diff-Quick at 2 h (E), 8 h (F), and 24 h (G) after aerosolization with LPS or at 24 h (H) after aerosolization with 0.9% saline are also shown. Photomicrographs are representative of five independent experiments; the brightness, contrast, and magnification of the pictures are similar. Five mice were used for each group. Original magnification, ×400. The graphs show the quantitation of total WBC (I) and neutrophil (J) counts in BALF. Five mice were tested for each group, and significance was calculated by a one-way analysis of variance (ANOVA). Values that were significantly different between the LPS- and saline-treated groups (P < 0.05) are indicated by asterisks.