FIG. 1.

Cell surface TLR9 is expressed on human tonsil cells. (A) A representative dot plot displays forward and side scatters of the tonsil cells used for live-cell gating (R1). (B) Staining with the TLR9 MAb (dark open line) is shown overlaying staining observed with the isotype control MAb (gray filled line). R2 indicates the cell surface TLR9⁺ cells. Dot plots of HLA-DR (MHC class II) and CD19 expression are shown for (C) the total live R1 gated cells and (D) the TLR9⁺ cells (R2 gate). Dead cells were excluded by 7AAD staining.