TABLE 2.
Kinetic analysis of mRNA expression in NALT after MALP-2 administrationa
| Timeb (h) | Relative expression (%)
|
||
|---|---|---|---|
| MIP-1αc | MIP-2c | MCP-1c | |
| 0 | NDd | ND | ND |
| 2 | ND | ND | ND |
| 6 | 0.95 | ND | ND |
| 16 | 1.10 | ND | 0.15 |
| 24 | 11.14 | 0.25 | 12.19 |
| 36 | ND | ND | ND |
a
MIP-1α, MIP-2, and MCP-1 were either not constitutively expressed in NALT or the basal transcription levels were below the detection limit.
b
Time after intranasal administration of MALP-2 (0.5 μg).
c
Results are expressed as the percentage with respect to the expression of the housekeeping gene β-actin. Values are means of at least two independent measurements.
d
ND, not detectable RT-PCR product.