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. 2016 Nov 14;8(2):114–122. doi: 10.1007/s13238-016-0340-z

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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miR-27b inhibitor promotes gastric cancer cell growth, migration, and invasion in vitro. (A) The effect on cell proliferation of miR-27b inhibitor in GES-1 cells was determined by the MTT assay. (B) Representative images show the colony formation of GES-1/miR-27b inhibitor and their control cells (left panel). Average colonies in each well for each group were counted from three independent experiments (right panel). (C) The effects of miR-27b inhibitor in GES-1 cells on cell migration and invasion were analyzed by Transwell migration and Matrigel-coated Transwell invasion analyses. (D) Q-RT analysis proliferation and metastasis genes. (E) Western blot analysis of proliferation and metastasis genes. *P < 0.05, **P < 0.01, ***P < 0.001