Skip to main content
. Author manuscript; available in PMC: 2018 Feb 2.
Published in final edited form as: Cell Stem Cell. 2016 Nov 17;20(2):261–273.e3. doi: 10.1016/j.stem.2016.10.004

Figure 4. Lipofibroblasts Give Rise to Activated Myofibroblasts during Fibrosis Formation.

Figure 4

(A) Schematic representation of the AdrpCre-ERT2 and mT/mG constructs.

(B) Timeline of tamoxifen and saline or bleomycin treatments. Mice were fed tamoxifen-containing pellets before saline or bleomycin was administered intratracheally. Lungs were harvested at 14 d.p.i.

(C–F) Immunofluorescent staining of saline-treated lungs showing DAPI, mGFP, and ACTA2 single channels in addition to a merged image.

(G) A high-magnification image of the region marked by the box in the merged image (F).

(H–K) Immunofluorescent staining of bleomycin-treated lungs showing DAPI, mGFP, and ACTA2 single channels in addition to a merged image.

(L) A high-magnification image of the region marked by the box in the merged image (K).

(M–O) Gating strategy for the detection of ACTA2+, LipidTOX+, and mGFP+ cell populations by FACS.

(P–V) FACS-based quantification of ACTA2+, mGFP+, and LipidTOX+ cell populations at 14 d.p.i.

(W–Y) qPCR for Acta2, Col1a1, and Fgf10 on mGFP+ cells sorted from saline- and bleomycin-treated lungs at 14 d.p.i.

Scale bar: 25 μm. SAL d14, n = 3; BLM d14, n = 3–4; n represents biological replicates. Data are presented as mean values ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.