TABLE 3.
Effect of oseltamivir carboxylate-rimantadine combinations on the yield of cell-associated influenza A/New Caledonia/20/99 (H1N1) virus in MDCK cells
| Concn of rimantadine (μM) | Inhibition of replication (%) with:a
|
||||
|---|---|---|---|---|---|
| Oseltamivir carboxylate at:
| |||||
| 0 μM | 0.001 μM | 0.01 μM | 0.1 μM | 1.0 μM | |
| 0 | NIb | NI | 20.0 ± 3.2 | 44.8 ± 2.2 | 66.7 ± 3.5 |
| 5 | 4.4 ± 3.4 | 5.3 ± 3.5f | 26.4 ± 3.9c,e | 47.0 ± 2.2c | 65.6 ± 3.3c |
| 20 | 9.3 ± 3.6 | 9.6 ± 3.6f | 35.1 ± 2.5c,f | 45.8 ± 2.7d | 70.4 ± 3.5d |
| 80 | 20.1 ± 4.0 | 24.5 ± 2.3f | 38.0 ± 2.5d,f | 55.1 ± 3.0d,f | 79.5 ± 2.4d,f |
a
Cell-associated virus yield was measured in MDCK cells by microneutralization followed by ELISA. Percent inhibition of virus replication was based on absorbance at 490 nm; control wells without drug were considered to have 0% inhibition. Each value is the mean ± SE for three independent experiments.
b
NI, no inhibition.
c
P was <0.05 compared with rimantadine used alone.
d
P was < 0.01 compared with rimantadine used alone.
e
P was < 0.05 compared with oseltamivir carboxylate used alone.
f
P was < 0.01 compared with oseltamivir carboxylate used alone.